

SYMPOSIUM  ORIGINAL RESEARCH 



J Pathol Inform 2011,
2:8 
Global error minimization in image mosaicing using graph connectivity and its applications in microscopy
Parmeshwar Khurd^{1}, Leo Grady^{1}, Rafiou Oketokoun^{2}, Hari Sundar^{1}, Tejas Gajera^{2}, Summer GibbsStrauss^{2}, John V Frangioni^{2}, Ali Kamen^{1}
^{1} Siemens Corporation, Corporate Research, Princeton, NJ, USA ^{2} Beth Israel Deaconess Medical Center, Boston, MA, USA
Date of Submission  20Oct2011 
Date of Acceptance  20Oct2011 
Date of Web Publication  19Jan2012 
Correspondence Address: Parmeshwar Khurd Siemens Corporation, Corporate Research, Princeton, NJ USA
Source of Support: None, Conflict of Interest: None  Check 
DOI: 10.4103/21533539.92039
Abstract   
Several applications such as multiprojector displays and microscopy require the mosaicing of images (tiles) acquired by a camera as it traverses an unknown trajectory in 3D space. A homography relates the image coordinates of a point in each tile to those of a reference tile provided the 3D scene is planar. Our approach in such applications is to first perform pairwise alignment of the tiles that have imaged common regions in order to recover a homography relating the tile pair. We then find the global set of homographies relating each individual tile to a reference tile such that the homographies relating all tile pairs are kept as consistent as possible. Using these global homographies, one can generate a mosaic of the entire scene. We derive a general analytical solution for the global homographies by representing the pairwise homographies on a connectivity graph. Our solution can accommodate imprecise prior information regarding the global homographies whenever such information is available. We also derive equations for the special case of translation estimation of an XY microscopy stage used in histology imaging and present examples of stitched microscopy slices of specimens obtained after radical prostatectomy or prostate biopsy. In addition, we demonstrate the superiority of our approach over treestructured approaches for global error minimization. Keywords: Image mosaicing, Wholeslide scanning in digital pathology, Graph Connectivity
How to cite this article: Khurd P, Grady L, Oketokoun R, Sundar H, Gajera T, GibbsStrauss S, Frangioni JV, Kamen A. Global error minimization in image mosaicing using graph connectivity and its applications in microscopy. J Pathol Inform 2011;2, Suppl S1:8 
How to cite this URL: Khurd P, Grady L, Oketokoun R, Sundar H, Gajera T, GibbsStrauss S, Frangioni JV, Kamen A. Global error minimization in image mosaicing using graph connectivity and its applications in microscopy. J Pathol Inform [serial online] 2011 [cited 2019 Nov 21];2, Suppl S1:8. Available from: http://www.jpathinformatics.org/text.asp?2011/2/2/8/92039 
1. Introduction   
Several applications such as multiprojector displays, underwater seafloor mapping, microscopy, etc., require the mosaicing of images (tiles) acquired by a camera as it traverses an unknown trajectory in 3D space. A homography relates the image coordinates of a point in each tile to those of a reference tile provided the imaged 3D scene is planar. ^{[1]} One approach ^{[2]} in such applications is to first perform pairwise alignment of the tiles that have imaged common features (points) in order to recover a homography relating the tile pair. We then find the global set of homographies relating each individual tile to a reference tile such that the homographies relating all tile pairs are kept as consistent as possible. Using these global homographies, one can generate a mosaic of the entire scene. The present paper is concerned with a method to compute the global homographies from the pairwise homographies via global error minimization (GEM) under the assumption that the 3D object being imaged is planar. We first describe our algorithm in the general case in section 2 and then describe a specific solution for microscopy in section 3. We demonstrate the performance of our algorithm on real microscopy data in section 4 and conclude with a discussion in section 5. Our contributions in this paper are threefold: (1) We provide a novel objective function and error minimization technique for global homography estimation in section 2 that can accommodate prior information regarding the global homographies whenever such information is available. (2) For the previously investigated case of global translation estimation in microscopy, we provide novel techniques to incorporate prior information. (3) We demonstrate the reduced error of the GEM techniques with respect to treestructured approaches in section 4 (Results) and also consider application of the general solution in section 2 for the microscopy application.
Relationships between our algorithms and the existing literature are pointed out in sections 1.1 and 3. Applications of image mosaicing algorithms to histology are separately discussed in section 1.2, wherein we also discuss our primary applications, namely histopathological imaging after radical prostatectomy and prostate biopsy.
1.1. Related Algorithms
Several existing approaches have focused on fast incremental mosaicing by imposing a suitable treestructure on the pairwise homography estimates. ^{[3],[4],[5],[6]} These approaches do not try to minimize global mosaicing error by integrating information from all pairwise estimates. To our knowledge, the only techniques attempting to reduce a global measure of mosaicing error. ^{[7],[8],[9],[10],[11],[12],[13]} However, the global sum of Euclidean distance errors between common feature points across image pairs is considered ^{[9],[10]} rather than our metric in (1), whereas Kang E, ^{[7]} uses the sum of intensity errors between such feature points as their metric. The homogeneous nature of microscopic tissue, e.g., lumen or stroma, can make it difficult to identify a sufficient number of common salient feature points for such approaches, rendering the metrics ^{[7],[9],[10]} harder to use. The focus is on reducing the cycle error from the pairwise 3D rotations and translations (they do not consider homographies) by back propagating it into the individual pairwise estimates. ^{[11]} Unlike us, they do not try to estimate homographies with respect to a single reference. The mosaic is built incrementally and translation and rotation errors in cycles are corrected whenever a cycle is detected. ^{[12]} However, this approach is not guaranteed to minimize a global error metric. The work ^{[8],[13]} does minimize a global error metric and has inspired us to extend their approach in several ways. Note that they only address the case of translation estimation discussed in section 3 and we additionally present our generalization to global homography estimation. The work ^{[13]} uses the same objective function for GEM as in (5), but without the prior information.The work ^{[8]} uses the SVD to solve a linear system involving the edgenode incidence matrix discussed in section 3, but this is equivalent to the solution in Preibisch S et al. ^{[13]} Neither Emmenlauer M et al ^{[8]} nor Preibisch S et al ^{[13]} present our alternative technique with prior information or discuss the connections with the graph Laplacian and the related efficient linear system solver presented in section 3.
1.2. Applications of Image Mosaicing Algorithms in Histology and Prostate Histopathology
As discussed in section 1.1, the work ^{[8],[13]} applied GEM techniques for 3D translation estimation in microscopy. That work was applied for 3D stitching of confocal microscopy images in nonpathological histology applications such as zebrafish embryo development, ^{[8]} cerebellum imaging of transgenic mice, ^{[13]} and central nervous system imaging in drosophila. ^{[13]} The work ^{[6]} mentioned above was concerned with 2D translation estimation in generic hematoxylin and eosin (H and E) stained histopathological imaging,whereas the work ^{[5]} was concerned with 2D rigid motion estimation for a variety of histological applications (both pathological and nonpathological) involving in vivo fibered microscopy videos.
The major clinical motivation for developing our novel mosaicing system for the assembly of wholeslide histopathological scans is the ability to utilize digital zoom in the context of feature recognition for the rapid automated prediagnosis of disease with the ability for pathologists to confirm suspicious areas rapidly. We have investigated two usage scenarios for our novel mosaicing algorithms. The first use case involves the online stitching of image tiles obtained by scanning a clinical histopathology slide using a microscope equipped with a motorized stage. ^{[14]} We have put our algorithm to this particular use as part of a specialpurpose wholeslide scanner being developed in our laboratory for optical imaging in both the visible and nearinfrared (NIR) spectra. However, the current computational implementation of our algorithm is suboptimal and hence slow, for the reasons described in section 4. Nevertheless, we are currently using it for prostate histopathology imaging, as described below, as part of a clinical research study. Our wholeslide scanner design falls under the progressive tile scanning category, and hence our image mosaicing algorithm can also be incorporated into other scanners that are similarly designed. ^{[14]} However, it is not useful in designs employing linescanning cameras (that are currently limited to optical imaging in the visible spectrum), such as those manufactured by Aperio. ^{[14]} The second use case involves the offline correction of mosaics that have been generated by other techniques used in other tilebased wholeslide scanners, and that have stitching errors. The correction of stitching errors from wholeslide scanners is not a major requirement in most applications, but apart from being visually disturbing, such errors can adversely affect the performance of automated image analysis algorithms in niche applications such as rare event counting, e.g., the counting of mitotic events in breast cancer histopathological imaging. However, we shall focus on the first use case in the remainder of this paper.
Our fully automated image mosaicing system employing the global energy minimization algorithm described in this paper has been used to yield qualitatively pleasing light microscopy mosaics of several 10× images of radical prostatectomy specimens and 40× images of prostate biopsy specimens. However, we do not address the specific problem of stitching quadrant prostatectomy specimens, which have specific problems associated with tearing and folding of tissue and require manual annotation of landmark points. ^{[15]} Our algorithm has only been used to stitch entire nonquadrant prostatectomy specimens acquired via a special microtome and processing techniques. However, it could potentially be used to stitch the individual quadrants in quadrant prostatectomy as well.
We also note that our prostate scanning procedures use an autofocusing technique, wherein the stage moves in the Zdirection for each tile to adjust focus while acquiring different tiles by moving in the XY plane. Therefore our stage motion differs slightly from the 2D translations considered. ^{[6]} and provided the motivation for developing our homographybased GEM algorithm in section 2. However, the comparison between the general homography model and the 2D translation model in section 4.3 shows that we can obtain very good stitching results solely with the 2D translation model and that the scaling issues can be ignored in our datasets.
2. Mosaicing Via Global Error Minimization Using Graph Connectivity: General Case   
Let us assume that N tiles were acquired by the camera. Mathematically, the global homography for tile i can be expressed as ^{[1]} . Here K denotes the intrinsic camera matrix, ^{[1]} R_{i} denotes the rotation of the camera axis of tile i with respect to a reference tile, p_{i} denotes the translation of tile i's center with respect to a reference tile's center, n and d_{i} are the normal vector of the plane and the distance to the plane respectively. We can form a connectivity graph between all acquired images (tiles) by considering each tile, with its associated global homography, as a node, and by placing an edge between tiles that share a border to have some overlap. Let N(i) denote the set of tiles that share any overlap with tile i. Let M_{ij} denote the pairwise homography between tiles i and j found via pairwise alignment and let the weight w_{ij} denote a measure of confidence in this pairwise estimate. To determine the global homographies, we need to minimize the following objective:
We take the squared distance between any two homography matrices A and B as , i.e., the squared Frobenius norm of AB, although alternative matrix distance measures such as those based upon the LogEuclidean transform or the BakerCampbellHausdorff formula may also be used for special cases involving positivedefinite homographies. ^{[5]} Note that it is desirable to use to symmetric distance measures s.t. , a condition that is satisfied by the squared Frobenius norm.
In certain instances, a noisy estimate M_{i} of the global homography for each tile may be available as prior information, e.g., from an electromagnetic tracking sensor. In such situations, we prefer to minimize the alternative objective function:
In Equation (2), the weight λ controls the tradeoff between the usages of the information in the pairwise estimates and the prior information. The above objective can be interpreted from a Bayesian viewpoint with the prior information corresponding to a uniform Gaussian prior probability. Strictly speaking, since matrices are derived from sensors during acquisition, they represent side information rather than prior knowledge. We are not restricted by symmetry considerations in choosing the distance measure for the prior information terms, but we continue to use the Frobenius norm.
The unique minimum of the objective function in Equation (2) can be found by setting its gradient to zero. The gradient of the objective function in Equation (2) with respect to the homography matrix M_{i} is
Note that
Setting the gradient in Equation (3) to zero, we get the following set of N simultaneous matrix equations:
The equations in (4) imply that each global homography matrix can be obtained by updating the prior estimate with a linear combination (with matrixvalued coefficients involving the unknown global homography matrices) of the neighboring global homography matrices. Each matrix equation in (4) is equivalent to a set of nine scalar equations for the individual matrix elements. We can concatenate the (a,b)^{th} elements M_{i} of the set of the 3×3 matrices M_{i} into a vector x and then compute this vector by solving the linear system Ax = y , where A is a 9N × 9N matrix . The exact form of A and y is given in Appendix A.
In case prior information is not available, we assume that a reference homography M_{N} is known and solve for the remaining N1 homographies in (4) with λ = 0. Note that knowing a singlereference homography may not guarantee a unique solution to these matrix equations, especially when the connectivity graph is disconnected. We shall revisit this issue in the case of translation estimation in section 3. For the case of a singlereference homography M_{N}, the matrix A in the linear system now becomes a 9(N  1) × 9(N  1) matrix and details are again provided in Appendix A.
Note that the above derivation assumes a general 3 × 3 homography matrix without any constraints on its form. However, general homography matrices have at most 8 degrees of freedom ^{[1],[16]} (up to scale), with 3 degrees of freedom each for the rotations and translations, and additional degrees of freedom arising from the variations in the intrinsic camera matrix during the scanning process, e.g., due to a changing focus. We can deal with this 8degree homography problem by setting M_{i} =1 for all homographies ^{[17]} and drop the corresponding rows and columns of A to get an updated 8(N 1) × 8(N 1) matrix for A. The vector y would also get updated with the appropriate terms transferred from the original LHS. Note that since M_{i} is nonzero for all tiles in our microscopy application (since the origin does not project to a line at infinity), we do not need to use the minimum norm constraints discussed. ^{[1]} In case the intrinsic matrix is fixed, but both translations and rotations are allowed, the homography matrix has the form M_{i} = (R_{i}  t_{i}n^{T}) and thus has only 6 degrees of freedom (the scalar factor d_{i} has been absorbed into t_{i}). In this case, using the method. ^{[12]} R_{i} and t_{i} can be recovered from the M_{i} found by the 8(N1) × 8(N1) linear system solver. For other special cases of homographies such as pure rotations (which would occur, e.g., when the camera is rotated from a singlevantage point to cover a large wall painting), we can impose these constraints on the solution found via the 8(N 1) × 8(N1) linear system solver using the SVDbased rotation extraction approach described. ^{[16]} However, pure rotations are not likely to happen in our microscopy applications. For the case of 2D translations considered in section 3, we obtain only two independent equations per matrix equation and do not need to impose any additional constraints on the resulting linear system solution, as demonstrated by the firstprinciples derivation in that section. In that section, we also describe how to deal with the case of multiple connected components in the graph connectivity (whenever they arise).
3. Microscopy XY Stage Motion Estimation and Image Mosaicing   
We now describe how our image mosaicing method from section 2 can be used in histological microscopic imaging. Since the motion reported by the slidescanning microscopy XY stage in histopathology imaging is typically unreliable, our imagebased mosaicing method is particularly useful in this application. Assuming that the microscope moves in a plane parallel to the planar specimen (we may assume this plane to be normal to the Zaxis) and K = I, the homography M_{i} thus reduces to . Then and the objective function to be minimized can be rephrased as
where p_{i} denote the coordinates available as prior information and p_{ij} denote the pairwise estimates. The new linear system to be solved in order to minimize (5), in lieu of (8), is derived below in Appendix A, and is much simpler.
Our method thus reduces to a simple three step noniterative approach similar: ^{[4],[10]} (1) Perform pairwise registration for all neighboring tiles by maximizing a similarity measure such as the normalized crosscorrelation (NCC) function. (2) Obtain absolute locations for each tile by solving a linear system such that the global measure of registration in (5) is minimized. Unlike, ^{[8],[13]} our method can accommodate imprecise prior information about the prior tile locations. (3) Perform blending to obtain a stitched image with nonoverlapping tiles.
We again present a firstprinciples derivation of the second step similar to the one in section 2. The tile connectivity and the homographies now have a special form. We describe the second step in detail in section 3 in order to point out the connections with the graph Laplacian and because our method of solution differs from the SVDbased solution reported. ^{[8]}
3.1. FirstPrinciples Derivation Based Upon the Graph Laplacian
Assuming a rectangular scan with R rows and C columns, we now form a graph with N = RC vertices with edge connectivity based upon eightconnected neighborhoods because only the eight nearest neighbors (NN) have overlapping regions. An example of this connectivity graph is shown in [Figure 1]a. We assume equal (unity) weights for all connections. Technically, we could choose weights based upon the NCC values, but we avoid doing this so that our solution is unaffected by inconsistencies in the NCC values across tile pairs. Such inconsistencies arise because NCC is not a true measure of pairwise registration accuracy, e.g., a given tile pair containing featureless regions has a high NCC value although the error in NCC peak estimation could be high on account of the relatively flat peak. In fact, evaluating registration accuracy is an important topic in its own right. ^{[18]} Note that we could have assigned smaller weights to the diagonal edges within the 8NN connectivity, but we do not do this because the diagonal input translations appear to be just as reliable as the vertical or horizontal translations.  Figure 1: (a) Connectivity graph, (b) global XY coordinates of the largest connected component, for a specimen comprised of 79×34 tiles
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The optimal locations for the tiles are found using a procedure similar to that in section 2. The partial derivative of the above objective in (5) w.r.t. p_{i} is
By setting this gradient to zero, we can see that the global position of each tile is the weighted sum of three terms, namely the average global positions of the neighboring tiles, the average of the pairwise neighboring translations and the prior position. Note that the X and Y coordinates are decoupled in this set of equations. If represents the concatenation of all the unknown Xcoordinates, we again need to solve a linear system Ax = y , the details for which are provided in Appendix A. The optimal Ycoordinates can also be obtained by solving a similar linear system. In case no prior information is available, and reference p_{N} is fixed to a known value, the dimension of x becomes (N1) and the resulting linear system is also provided in Appendix A.
The objective in Equation (5) is also decoupled in X, Y and the part corresponding to the Xcoordinates alone, Φ^{X} , can be rephrased in terms of the graph Laplacians and the edgenode incidence matrix. ^{[19]} The N×N connectivity graph Laplacian is defined as L = DW, where D is a diagonal matrix such that and W is a binary adjacency matrix such that W_{ij} = 1 if j ∈ N (i)and j≠i. Assuming N_{e} total edges in the graph, the N_{e} N edgenode incidence matrix E is defined to have the nonzero row entries E_{ki} = 1 and E_{kj} = 1 if i < j and j ∈ N_{i}. Note that L = E^{T}E. If t is the concatenation of all the N_{e} pairwise translations (Xcomponents), then
and the linear system in Equation (10) corresponds to . In the absence of prior information, the linear system corresponds to , where the matrix A is obtained by dropping the last row and column of L, F is obtained by dropping the column of E corresponding to the last tile and l_{N} denotes the vector of the first (N1) rows of the last column of L. We note that A = F^{T}F in this case. Since matrix (L + λI) is positive definite, ^{[19]} we can use the Cholesky decomposition or the preconditioned conjugate gradient (PCG) algorithm to solve the corresponding linear system. When λ is large (indicating more confidence in the prior information), fewer PCG iterations are required for convergence.
With full 8NN connectivity (as described above), in the absence of prior information, matrix A of dimension (N  1) × (N  1) is invertible because we include the Nth tile in the neighborhood connectivity. Also, note that this linear system can be easily modified to accommodate holes (i.e., constant intensity tiles which cannot be aligned via pairwise estimation) within the specimen. We simply drop the corresponding images, appropriately change the 8NN connectivity and modify the matrix A and the column vector y . If the holes end up disconnecting the specimen, then we need to supply reference pixel coordinates for each connected component and solve one linear system per component. ^{[19]} Note that we can also accommodate partial holes that only remove a part of the 8NN connectivity. Using W, we can find all connected components in N steps using depthfirstsearch or breadthfirstsearch (regardless of the actual number of components) and compute the matrices A^{c} corresponding to each component. Since each matrix A ^{c} is also positive definite, we can again use the Cholesky decomposition or the preconditioned conjugate gradient algorithm to solve the corresponding linear system. For each connected component, we select a reference tile and use the following technique to pick reference tile coordinates: We specify the reference coordinates for only one component (the largest one) using the XY stage report and use linear extrapolation in order to propagate reference coordinates from the largest component to all others. This choice is motivated by the need for automation and the various stage error considerations discussed in section 4.
4. Results and Discussion   
Our microscopy data consists of hematoxylin and eosin (H and E) stained images of sliced prostate specimens imaged with a light microscope after radical prostatectomy or after a prostate biopsy. After radical prostatectomy, each image tile was acquired using a Nikon Eclipse TE2000 microscope equipped with a Velmex BiSlider motorized stage at 10× resolution with 0.64 micron pixel size and was of size 1392 × 1040 pixels. In addition to problems associated with CCD/stage misalignment and backlash, this Velmex stage has a repeatability error of 5 microns, which translates to roughly 8 pixels at 10×. Following prostate biopsy, each image tile was acquired using a Nikon Eclipse 55i microscope equipped with a PRIOR H101A motorized stage at 40× resolution with 0.16 micron pixel size and was of size 1360 × 1024 pixels. This PRIOR stage does not suffer from backlash problems, but we still have some irremovable CCD/stage misalignment and a repeatability error of 0.75 microns, which translates to roughly 5 pixels at 40×. For pairwise homography estimation, the translation estimate found via NCC was used to identify rough correspondences which were then refined using local normalized crosscorrelation and subsequently used for homography estimation.
4.1. Global Translation Estimation Results on Radical Prostatectomy Data
[Figure 1] shows the global positions computed using the GEM algorithm in section 3 without any prior information, for the largest component of a specimen comprised 79 × 34 tiles that contained both partial and full holes. On account of the holes, only 7942 pairwise translations out of a total possible 11207 8NNconnectivity edges were fed to our system and our system first eliminated more than 50 tiny components. Note the missing tiles (holes) in the center of the specimen in [Figure 1]. Also note the slight tilt in the Xcoordinates possibly on account of the backlash that occurs when the XY stage returns to its leftmost Xcoordinate after scanning an entire row. This indicates imperfect backlash correction by our stage. Part of this tilt is due to CCD/stage misalignment as well. The complete stitched image is shown in [Figure 2]. We currently observe a runtime of a few seconds on 79 × 34 images (tiles) for the global registration and a runtime of around 1 hour to obtain all pairwise translations. Therefore, speed is not a critical issue for the global registration. The net computation time of about 1 hour for the global coordinates calculation is reasonable for this application since this acquisition also required about 1 hour (on account of our autofocusing procedure) and the subsequent mosaic pyramid creation also required about an hour (on account of the large image sizes). In addition, since the pairwise registration step is highly parallelizable on account of registrations being independent, greater speedup can be obtained by using more processor cores. Moreover, the pairwise registration step can be simultaneously performed during the hourlong acquisition thus significantly reducing the net computation time for calculation of global coordinates. Since each image tile needs to be registered to four previously acquired tiles (except for tiles located on some of the borders), the parallelization potential is present even when the pairwise registration is performed in conjunction with acquisition. Additionally, individual pairwise registrations can themselves be sped up, e.g., via parallelization on GPU's (graphical processing units).  Figure 2: Stitched image of the largest connected component from the 79×34 tiles in Fig. 1
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4.2. Comparison of Methods on Simulated Data
We conducted an experiment with noisy simulated translations (not derived from images) on a 3 × 3 mosaic in order to confirm the errorminimizing advantages of our approach with respect to treestructured approaches [Figure 3]. We considered equispaced square tiles with 10% overlap and added independent zeromean Gaussian noise (σ_{t} =2% of the square image tile size) to each coordinate of the 20 pairwise translations. We chose the central tile as the reference tile for our GEM approach as it minimized trace (A ^{1}) and hence the positioning error in [Table 1]. When averaged over 5000 runs, we obtained an average positioning error of 1.34% [row 5 of the Table in [Figure 3]] with our GEM approach using the central (5th) tile. For comparison, we also show the average positioning error corresponding to tiles 1 (left top) and 2 (middle top) in rows 3 and 4 respectively. This central tile is also the optimal reference for the treestructured approaches considered below. We obtained an error and 2.70% (row 1) with the fishbone approach. ^{[3]} Since we allow diagonal connections and each connection is associated with the same noise level, the star graph (rather than the fishbone graph) is the ideal tree for our connectivity graph because it minimizes the sum of all path lengths from the reference node to all other nodes. The star tree yielded an average positioning error of 2.23% (row 2). The mean sum of the squared positioning errors (last column) for the GEM approaches (rows 35), the fishbone approach and the star approach assumed their expected values: , respectively, in this simulation. The GEM approach (rows 35) clearly reduces the overall positioning error although the need to obtain all pairwise translations increases its computation time. In the last two rows, we also show the results using prior information. Zeromean Gaussian noise with σ_{p} =2% and 4% was added to the X, Y coordinates of the true locations and used as prior information in rows 6 and 7, respectively. The parameter λ was set to 8 and 32 in rows 6 and 7, respectively, which represented nearoptimal values in this simulation. The squared error with prior information as precise as the translation estimates (row 7) was greatly superior to the best result without prior information (row 5) and even with very noisy prior information, the advantage of using prior information persisted (row 6).  Figure 3: (a) 3 x 3 connectivity graph used during global error minimization, (b) Star graph (optimal tree, please see text for explanation), (c) Fishbone graph^{[3]}
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 Table 1: Positional errors for different global position estimators (GEM1, GEM2, GEM5 represent our global error minimization technique without prior information with tiles 1, 2, and 5 as references and GEMP represents our GEM technique with prior information)
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4.3. Comparison of Methods on H and E Data
We shall now compare our methods on H and E microscopy images acquired with motorized stages yielding different levels of accuracy in the prior coordinates. Unlike section 4.2, we no longer possess knowledge of the true global coordinates, i.e., the ground truth. We start by presenting a 40× cutout partial mosaic from a prostate biopsy specimen obtained with a newlypurchased, freshly calibrated, encoded XY stage (PRIOR H101A) providing extremely reliable prior information. There was a 15% overlap between neighboring tiles during acquisition. There was no backlash with this stage, but we note that there was an alignment error between the camera's CCD and the XY stage motion which could not be easily removed by rotating the camera on account of camera rotation accuracy limitations. We estimated the true motions in the X and Y directions by performing multiple pairwise registrations and subsequently using a linefitting procedure. The beneficial effect of using the corrected vs. the original prior coordinates is shown in [Figure 4]. (Please see insets 4(c)(f) of highlighted areas to notice the presence of the seams of the tiles in [Figure 4]a and their absence in [Figure 4]b. We note that there were no visible stitching errors within this entire mosaic consisting of 72 × 168 tiles. Given this accurate corrected prior information, there is no need to use our GEM algorithms. It also indicates that the Zmotion due to autofocusing can be ignored during stitching.  Figure 4: Precise prior information: (a) Translationbased mosaic stitched using the original prior coordinates, (b) Translationbased mosaic stitched using the corrected prior coordinates, (c) Red inset from 4(a), (d) Yellow inset from 4(a)
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We now present a 10× partial mosaic from a radical prostatectomy specimen acquired with an inexpensive stage (Velmex BiSlider) providing highly unreliable prior information, with 20% overlap between neighboring tiles. In [Figure 5], we show a comparison of a partial 2 × 3 mosaic, independently stitched from the corresponding 2 × 3 tiles, via homography estimation and pure translation estimation with and without prior information. The prior information was obtained via line fitting [Figure 4] and linear extrapolation to coordinates obtained without prior information and was hence biased and not very reliable. As seen in this example, the differences between our three GEM approaches on this partial dataset are not very significant. [Figure 5]c shows extremely minor errors (please see inset 5ef of yellow highlighted areas) although we set λ to 2.0 (a lower value than any diagonal Laplacian entry), indicating the need for more reliable prior information. By manual placing landmarks, we obtained ground truth translations between neighboring pairs and compared the results of our automatic pairwise alignment procedure with the ground truth. The median (maximum) Euclidean pairwise alignment error for the 11 2D translations was 1.0 (3.6) pixels, indicating nearperfect pairwise alignment. Some of the homography matrices indicate that the XY stage motion does not seem to be perfectly parallel to the plane of the specimen. However, we have observed our pairwise homography estimation to be less stable (please see errors in insets 5gh of red highlighted area in [Figure 5]d than the pairwise translation estimation on account of the small overlap and the occasional almost featureless overlapping regions and are currently in the process of improving our pairwise homography estimation algorithm. Moreover, at this moment, our visualization software builds multiresolution pyramids computed from 2D tile coordinates. Therefore, we have not attempted to stitch a full slide using homographies as in [Figure 2]. Note that in [Figure 5], we have used image replacement rather than blending in order to highlight the differences between the different GEM approaches.  Figure 5: Imprecise prior information: (a) Neighboring 2x3 image tiles (b) Translationbased mosaic stitched using our GEM approach without prior information (Sec. 3) (c) Translationbased mosaic using our GEM approach with prior information (Sec. 3) (d) Homographybased mosaic stitched using our GEM approach without prior information (Sec. 2) (e) (e) Yellow inset from 5(b) (f) Yellow inset from 5(c) [The difference between (e) and (f) is minor, but note that the size of the central purple nucleus slightly reduces in (f)] (g) Red inset from 5(b) (h) Red inset from 5(d)
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Finally, in [Figure 6], we present an example of a 40× cutout partial mosaic from a prostate biopsy specimen (56 × 156 tiles) obtained with the same reliable PRIOR stage, as in [Figure 4], but after several months of usage. There was a 10% overlap between neighboring tiles during acquisition. The prior information was no longer as reliable and the benefit of using our 2D translationbased GEM algorithm with a prior weight of 0.01 is clearly visible in [Figure 6]. According to the manufacturer, this stage does not require any recalibration procedure and the exact cause for this drop in accuracy with respect to [Figure 4] is not yet clear. Nevertheless, given the fact that the quoted repeatability error of this stage is roughly 5 pixels, we cannot always expect the nearperfect scans corresponding to [Figure 5].  Figure 6: Semiprecise prior information: (a) Translationbased mosaic stitched using the corrected prior coordinates (b) Translationbased mosaic using our GEM approach with prior information (Sec. 3) (c) Yellow inset from 6(a) (d) Red inset from 6(a) (e) Yellow inset from 6(b) (f) Red inset from 6(b)
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We note that although the GEM algorithm without prior information could be readily applied to our radical prostatectomy data obtained with large overlaps (e.g., 20%), the use of GEM algorithms with prior information is more desirable for prostate biopsy scans with smaller overlaps (e.g., 10%). This is because biopsy scans with smaller overlaps lead to more errors in the pairwise alignment on account of the more frequent featureless (often nearly empty) overlapping regions. (We note that smaller overlaps are desirable on account of the resulting faster acquisition times.) Also, biopsy scans involve thin individual structures and contain more large connected components than radical prostatectomy specimens which typically contain only one or two large components.
5. Conclusion   
We have presented a novel general solution for image mosaicing using graph connectivity and demonstrated its applicability for homography estimation and 2D translation estimation in histological microscopy imaging. Our solution can accommodate imprecise prior information regarding the unknown homographies or translations whenever such information is available. The general solution of section 2 has been applied to the microscopy data in section 4 and the XY stage motion estimation algorithm from Section 3 has been used to stitch full slides obtained after radical prostatectomy. Note that the same graph connectivity plays an important role in the solutions for both the generic and the microscopy scenarios, as seen by equations (8) and (10). Through experiments with simulated data, we have shown that prior information can significantly improve the accuracy of the GEM approaches and have also shown that the GEM approaches are superior to treestructured approaches. We could also use an algorithm similar to the one in section 3 to compute 3D global positions from 3D translations as in the confocal microscopy volumetric stitching application considered in Ref. ^{[8]} We also note that having provided a general energy minimization framework incorporating prior information in equation (2), we could replace the Frobenius norm in equation (2) with outlierresistant norms and perform the optimization in an iterative manner. Another interesting avenue for future work would be to investigating the tradeoff between computational speed and accuracy in our GEM approach with prior information by selecting fewer pairwise connections, e.g., those corresponding to an optimal tree. This would reduce the number of pairwise registrations required and hence increase speed.
Acknowledgement   
This work was supported by grant 1R01CA13449301A1 from the NIH.
Appendix A   
The matrix A and vector y in the linear system corresponding to equation (4) are as follows:
When no prior information is available and M_{N} is known, we set λ to 0 in (8), the index i ranges from 1 to N1 and y becomes
The matrix A and vector y in the linear system corresponding to (6) are as follows:
When no prior information is available and is known, we set λ to 0 in (10), the index i ranges from 1 to N 1, and y becomes:
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[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6]
[Table 1]
