Journal of Pathology Informatics Journal of Pathology Informatics
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RESEARCH ARTICLE
Year : 2014  |  Volume : 5  |  Issue : 1  |  Page : 4

Color standardization in whole slide imaging using a color calibration slide


Department of Pathology, Massachusetts General Hospital, Boston, MA 02114, USA

Correspondence Address:
Pinky A Bautista
Department of Pathology, Massachusetts General Hospital, Boston, MA 02114
USA
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2153-3539.126153

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Background: Color consistency in histology images is still an issue in digital pathology. Different imaging systems reproduced the colors of a histological slide differently. Materials and Methods: Color correction was implemented using the color information of the nine color patches of a color calibration slide. The inherent spectral colors of these patches along with their scanned colors were used to derive a color correction matrix whose coefficients were used to convert the pixels' colors to their target colors. Results: There was a significant reduction in the CIELAB color difference, between images of the same H & E histological slide produced by two different whole slide scanners by 3.42 units, P < 0.001 at 95% confidence level. Conclusion: Color variations in histological images brought about by whole slide scanning can be effectively normalized with the use of the color calibration slide.


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