Close
  Indian J Med Microbiol
 

Figure 9: SIVQ-LCM of single cells. (a) An FOV of HandE of FFPE dog prostatic tissue on a membrane slide. A single vector was chosen (middle of figure) to identify single prostate luminal cells with prominent nucleoli and a glassy peri-nucleolar space (b). The paint was recognized by AutoScan, and a dissection map was created with laser shots that were manually placed within the map (c). The cells were dissected and the remaining tissue and map are shown in (d). The microdissected cells are shown in (e) where one can see 11 nuclei and their corresponding luminal cytoplasm. The remaining tissue is shown in (f)

Figure 9: SIVQ-LCM of single cells. (a) An FOV of HandE of FFPE dog prostatic tissue on a membrane slide. A single vector was chosen (middle of figure) to identify single prostate luminal cells with prominent nucleoli and a glassy peri-nucleolar space (b). The paint was recognized by AutoScan, and a dissection map was created with laser shots that were manually placed within the map (c). The cells were dissected and the remaining tissue and map are shown in (d). The microdissected cells are shown in (e) where one can see 11 nuclei and their corresponding luminal cytoplasm. The remaining tissue is shown in (f)