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  Indian J Med Microbiol
 

Figure 2: Reflective vs. image-based autofocus schemes. Reflective-based approaches set focus a fixed distance above the reference surface (glass slide surface). Image-based approaches sample images at several different z-planes and apply a figure of merit calculation to determine the optimal focal plane

Figure 2: Reflective vs. image-based autofocus schemes. Reflective-based approaches set focus a fixed distance above the reference surface (glass slide surface). Image-based approaches sample images at several different z-planes and apply a figure of merit calculation to determine the optimal focal plane