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  Indian J Med Microbiol
 

Figure 3: Image-based auto-focusing approaches. A section of tissue with green cross hairs representing the focus points used to calculate a focus map. The blue dotted line (focus map) is the calculated focal plane interpolated between focus points. Red boxes are the focal plane for each field of view and each one can be adjusted in the z-position during a scan. Line scanners have more ability to adjust the z-depth during scanning. Both line and tile scanners can incorrectly predict focus between focus points. Focusing on every tile increases chances of having correct focus throughout the scan

Figure 3: Image-based auto-focusing approaches. A section of tissue with green cross hairs representing the focus points used to calculate a focus map. The blue dotted line (focus map) is the calculated focal plane interpolated between focus points. Red boxes are the focal plane for each field of view and each one can be adjusted in the z-position during a scan. Line scanners have more ability to adjust the z-depth during scanning. Both line and tile scanners can incorrectly predict focus between focus points. Focusing on every tile increases chances of having correct focus throughout the scan