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  Indian J Med Microbiol
 

Figure 4: Predictive auto-focusing in IDS scanning. (a) An IDS scanning system consists of a two cameras, one of which is a high speed autofocus (AF) camera and the other of which is a high resolution imaging camera. A single optical path from the sample is split into two cameras by a beam splitter. (b) The focus sensor acquires three images in different z-planes. The system calculates the optimal focus position. Once positioned there, the main imaging camera takes a high resolution image of the sample. While the main imaging camera is reading out, the process is repeated. (c) Because the system is in continuous motion, the three AF images only have a small region of overlap. The system uses the overlapping region to calculate the best focal plane

Figure 4: Predictive auto-focusing in IDS scanning. (a) An IDS scanning system consists of a two cameras, one of which is a high speed autofocus (AF) camera and the other of which is a high resolution imaging camera. A single optical path from the sample is split into two cameras by a beam splitter. (b) The focus sensor acquires three images in different z-planes. The system calculates the optimal focus position. Once positioned there, the main imaging camera takes a high resolution image of the sample. While the main imaging camera is reading out, the process is repeated. (c) Because the system is in continuous motion, the three AF images only have a small region of overlap. The system uses the overlapping region to calculate the best focal plane