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  Indian J Med Microbiol
 

Figure 3: Extended focusing algorithm. This picture corresponds to the same area on the same slide as shown in Figure 2's multilayer stack. All areas are brought into improved focus; however, extracellular debris are accentuated (worse with increasing focal planes), and crisp detail seen in the multilayer stack is lost

Figure 3: Extended focusing algorithm. This picture corresponds to the same area on the same slide as shown in Figure 2's multilayer stack. All areas are brought into improved focus; however, extracellular debris are accentuated (worse with increasing focal planes), and crisp detail seen in the multilayer stack is lost