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  Indian J Med Microbiol
 

Figure 3: Segmentation of fluorescence digital tissue images. Slides were scanned at 20× magnification. TMA cores were analyzed using HCImage. (a) H and E kidney core; (b) Hoechst, NF-κ B (red), CD44 (green)-stained kidney core; (c) magnified view of kidney core; (d) detection of individual nuclei edges in kidney; (e) detection of cell edges in kidney; (f) cytoplasm and membrane mask in kidney; (g) H and E MiaPaca-2 core; (h) Hoechst, NF-κ B, CD44-stained MiaPaca-2 core; (i) magnified view of MiaPaca-2 core; (j) detection of individual MiaPaca-2 nuclei edges; (k) detection of MiaPaca-2 cell edges; (l) cytoplasm and membrane mask in MiaPaca-2 core. a-b and g-h are from same cores in different serial sections of the TMA

Figure 3: Segmentation of fluorescence digital tissue images. Slides were scanned at 20× magnification. TMA cores were analyzed using HCImage. (a) H and E kidney core; (b) Hoechst, NF-κ B (red), CD44 (green)-stained kidney core; (c) magnified view of kidney core; (d) detection of individual nuclei edges in kidney; (e) detection of cell edges in kidney; (f) cytoplasm and membrane mask in kidney; (g) H and E MiaPaca-2 core; (h) Hoechst, NF-κ B, CD44-stained MiaPaca-2 core; (i) magnified view of MiaPaca-2 core; (j) detection of individual MiaPaca-2 nuclei edges; (k) detection of MiaPaca-2 cell edges; (l) cytoplasm and membrane mask in MiaPaca-2 core. a-b and g-h are from same cores in different serial sections of the TMA