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  Indian J Med Microbiol
 

Figure 2: (a) Hematoxylin and eosin stained images are separated into (b) hematoxylin and (c) eosin channels via color deconvolution. The hematoxylin channel is thresholded to detect (d) centroids of individual cancer nuclei, which are used to construct (e), (h), (k) Voronoi diagram, (f), (i), (l) Delaunay triangulation, and (g), (j), (m) minimum spanning tree. The graphs are subsequently used to extract 50 features describing nuclear architecture. Note the variations in nuclear arrangement when exposing (e)-(g) large, (h)-(j) medium, and (k)-(m) small FOVs

Figure 2: (a) Hematoxylin and eosin stained images are separated into (b) hematoxylin and (c) eosin channels via color deconvolution. The hematoxylin channel is thresholded to detect (d) centroids of individual cancer nuclei, which are used to construct (e), (h), (k) Voronoi diagram, (f), (i), (l) Delaunay triangulation, and (g), (j), (m) minimum spanning tree. The graphs are subsequently used to extract 50 features describing nuclear architecture. Note the variations in nuclear arrangement when exposing (e)-(g) large, (h)-(j) medium, and (k)-(m) small FOVs