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  Indian J Med Microbiol
 

Figure 2: Image analysis using Genie pattern recognition technology. (1) Nestin IHC stained podocytes (a), allowed Genie to recognize glomeruli more specifically (b). The Glomeruli detection algorithm was then used as a classifier for subsequent analysis. The Nuclear algorithm was used to count nuclei in the classified regions (c). (2) Genie was used to identify glomeruli (a, b), which were then excluded from subsequent analysis. Non-glomerular nestin expression (DAB) was quantified by the Positive Pixel Count algorithm in the non-glomerular classifier (c). (3) Genie was trained to recognize dilated tubules on H&E stained kidney sections (a, b) and used them as a classifier for Positive Pixel Count algorithm. White pixels (tubular lumen) were counted (c). (4) Genie was trained to recognize mineral aggregates on H&E stained kidney sections (a, b) and used them as a classifier for Positive Pixel Count algorithm. Brown pixels (crystals) were counted (c). The pictures are displayed at following magnification: 1: 20×; 2 and 3: 6.5×; 4: 10×

Figure 2: Image analysis using Genie pattern recognition technology. (1) Nestin IHC stained podocytes (a), allowed Genie to recognize glomeruli more specifically (b). The Glomeruli detection algorithm was then used as a classifier for subsequent analysis. The Nuclear algorithm was used to count nuclei in the classified regions (c). (2) Genie was used to identify glomeruli (a, b), which were then excluded from subsequent analysis. Non-glomerular nestin expression (DAB) was quantified by the Positive Pixel Count algorithm in the non-glomerular classifier (c). (3) Genie was trained to recognize dilated tubules on H&E stained kidney sections (a, b) and used them as a classifier for Positive Pixel Count algorithm. White pixels (tubular lumen) were counted (c). (4) Genie was trained to recognize mineral aggregates on H&E stained kidney sections (a, b) and used them as a classifier for Positive Pixel Count algorithm. Brown pixels (crystals) were counted (c). The pictures are displayed at following magnification: 1: 20×; 2 and 3: 6.5×; 4: 10×