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  Indian J Med Microbiol
 

Figure 1: Comparative FFOCT and HandE-stained histology images from the testes of normal adult Sprague-Dawley rats, and rats exhibiting a sertoli-cell only phenotype. (a) Seminiferous tubules in the testis of a normal rat. The tubules are relatively uniform in size and shape (diameter 328 ± 11 μm). (b) Same specimen processed and stained for conventional (HandE) histology. Arrows point to the sperm within the tubule lumen. (c) Seminiferous tubules in the testis of a rat treated with busulfan. Tubules, on average are thinner, and show a greater degree of heterogeneity in size and shape (diameter 178 ± 35 μm). Only ~10% of the tubules show normal spermatogenesis as identified by presence of sperm tails (bright white hair-like structures within the lumen; long arrows). The remainder of the tubules showed no sperm within the lumen (short arrows). (d) HandE staining of the same specimen confirms the observations. Long and short arrows point to tubules with and without spermatogenesis, respectively. Field of view in each panel: 1 mm2

Figure 1: Comparative FFOCT and HandE-stained histology images from the testes of normal adult Sprague-Dawley rats, and rats exhibiting a sertoli-cell only phenotype. (a) Seminiferous tubules in the testis of a normal rat. The tubules are relatively uniform in size and shape (diameter 328 ± 11 μm). (b) Same specimen processed and stained for conventional (HandE) histology. Arrows point to the sperm within the tubule lumen. (c) Seminiferous tubules in the testis of a rat treated with busulfan. Tubules, on average are thinner, and show a greater degree of heterogeneity in size and shape (diameter 178 ± 35 μm). Only ~10% of the tubules show normal spermatogenesis as identified by presence of sperm tails (bright white hair-like structures within the lumen; long arrows). The remainder of the tubules showed no sperm within the lumen (short arrows). (d) HandE staining of the same specimen confirms the observations. Long and short arrows point to tubules with and without spermatogenesis, respectively. Field of view in each panel: 1 mm<sup>2</sup>