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  Indian J Med Microbiol
 

Figure 1: Multiplexed panels of tissue system biomarkers. (a-d) Ki-67 (green), cytokeratin 20 (red), beta-catenin (yellow) and overlay of Ki-67, cytokeratin 20 and beta-catenin. (e-h) p16 (green), alpha-methylacyl-coenzyme A racemase (red), p53 (yellow) and overlay of p16, alpha-methylacyl-coenzyme A racemase and p53. (i-l) human epidermal growth factor receptor 2/neu (green), cytokeratin 20 (red), CDX-2 (yellow) and overlay of human epidermal growth factor receptor 2/neu, cytokeratin 20 and CDX-2. Panels (m-p) CD68 (green), nuclear factor kappa-B p65 (red), COX-2 (yellow) and overlay of CD68, nuclear factor kappa-B and COX-2. (q-t) hypoxia-inducible factor-1 alpha (green), CD45RO (red), CD1a (yellow) and overlay of hypoxia-inducible factor-1 alpha, CD45RO, and CD1a. Hoechst labeling is shown in blue

Figure 1: Multiplexed panels of tissue system biomarkers. (a-d) Ki-67 (green), cytokeratin 20 (red), beta-catenin (yellow) and overlay of Ki-67, cytokeratin 20 and beta-catenin. (e-h) p16 (green), alpha-methylacyl-coenzyme A racemase (red), p53 (yellow) and overlay of p16, alpha-methylacyl-coenzyme A racemase and p53. (i-l) human epidermal growth factor receptor 2/neu (green), cytokeratin 20 (red), CDX-2 (yellow) and overlay of human epidermal growth factor receptor 2/neu, cytokeratin 20 and CDX-2. Panels (m-p) CD68 (green), nuclear factor kappa-B p65 (red), COX-2 (yellow) and overlay of CD68, nuclear factor kappa-B and COX-2. (q-t) hypoxia-inducible factor-1 alpha (green), CD45RO (red), CD1a (yellow) and overlay of hypoxia-inducible factor-1 alpha, CD45RO, and CD1a. Hoechst labeling is shown in blue