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  Indian J Med Microbiol
 

Figure 2: Focused and defocused images of the same nucleus (a and c), as well as plots of their gray images (b and d). The black line is the contour found by the segmentation algorithm

Figure 2: Focused and defocused images of the same nucleus (a and c), as well as plots of their gray images (b and d). The black line is the contour found by the segmentation algorithm