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  Indian J Med Microbiol
 

Figure 2: Multiplexed immunofluorescence image of a tissue microarray spot. (a) Pseudocolored multi-channel fluorescence image is shown for the estrogen receptor(+) invasive ductal carcinoma spot 55 of the tissue microarray [Table 1]. Human epidermal growth factor 2 is shown in red, estrogen receptor in blue, and progesterone receptor in green (see legend in the top left corner). Areas of progesterone receptor/estrogen receptor coexpression will appear in cyan, human epidermal growth factor 2/estrogen receptor co-expression in magenta, and progesterone receptor/human epidermal growth factor 2 coexpression in yellow. Arrows point to three different heterogeneous regions in the tumor sample, with varying populations of estrogen receptor(+), progesterone receptor(+), and estrogen receptor(+)/progesterone receptor(+) cells. The upper arrow indicates a tumor microdomain with higher than average estrogen receptor(+)/progesterone receptor(+) phenotyped cells. The middle arrow indicates a microdomain probably the best representative of the tumor sample en masse, containing mostly estrogen receptor(+) cells. In the third microdomain, indicated by the lower arrow, there is a higher than average population of progesterone receptor(+) cells. (b-d) Individual pseudocolored fluorescence images are shown for human epidermal growth factor 2, estrogen receptor, and progesterone receptor

Figure 2: Multiplexed immunofluorescence image of a tissue microarray spot. (a) Pseudocolored multi-channel fluorescence image is shown for the estrogen receptor(+) invasive ductal carcinoma spot 55 of the tissue microarray [Table 1]. Human epidermal growth factor 2 is shown in red, estrogen receptor in blue, and progesterone receptor in green (see legend in the top left corner). Areas of progesterone receptor/estrogen receptor coexpression will appear in cyan, human epidermal growth factor 2/estrogen receptor co-expression in magenta, and progesterone receptor/human epidermal growth factor 2 coexpression in yellow. Arrows point to three different heterogeneous regions in the tumor sample, with varying populations of estrogen receptor(+), progesterone receptor(+), and estrogen receptor(+)/progesterone receptor(+) cells. The upper arrow indicates a tumor microdomain with higher than average estrogen receptor(+)/progesterone receptor(+) phenotyped cells. The middle arrow indicates a microdomain probably the best representative of the tumor sample en masse, containing mostly estrogen receptor(+) cells. In the third microdomain, indicated by the lower arrow, there is a higher than average population of progesterone receptor(+) cells. (b-d) Individual pseudocolored fluorescence images are shown for human epidermal growth factor 2, estrogen receptor, and progesterone receptor