Close
  Indian J Med Microbiol
 

Figure 7: Quadratic entropy implementation with patient and core-level results. (a) The estrogen receptor immunofluorescence image is shown for spot 5. Note different levels of estrogen receptor signal intensity throughout the tumor sample. (b) Regions of interest are selected via k-means clustering on the XY coordinates of each cell (although one may also select regions of interests manually). (c) The frequencies of each estrogen receptor-intensity species (where 1 is lowest intensity and 4 is highest) are shown for spot 5 by the blue bars. The species frequencies for spot 76 (immunofluorescence and regions of interests not shown) are shown in red to provide another example of potential estrogen receptor-intensity distributions. Quadratic entropy is reported for the entire tumor sample (HETslide), as well as mean, minimum, and maximum quadratic entropy of each regions of interest (HETROI). (d and e) Quadratic entropy was calculated for patients AL13-3 and AL13-14, from cohorts estrogen receptor(+) invasive ductal carcinoma and estrogen receptor(−) invasive ductal carcinoma, respectively. HETslide is computed for each individual replicate core of each patient and then computed for the entire patient (consolidating the cells from each core). HETROI is computed for each region of interest in the cores and the mean, minimum, and maximum HETROI scores are reported for each core and patient

Figure 7: Quadratic entropy implementation with patient and core-level results. (a) The estrogen receptor immunofluorescence image is shown for spot 5. Note different levels of estrogen receptor signal intensity throughout the tumor sample. (b) Regions of interest are selected via</b> <i>k</i>-means clustering on the XY coordinates of each cell (although one may also select regions of interests manually). (c) The frequencies of each estrogen receptor-intensity species (where 1 is lowest intensity and 4 is highest) are shown for spot 5 by the blue bars. The species frequencies for spot 76 (immunofluorescence and regions of interests not shown) are shown in red to provide another example of potential estrogen receptor-intensity distributions. Quadratic entropy is reported for the entire tumor sample (HETslide), as well as mean, minimum, and maximum quadratic entropy of each regions of interest (HETROI). (d and e) Quadratic entropy was calculated for patients AL13-3 and AL13-14, from cohorts estrogen receptor(+) invasive ductal carcinoma and estrogen receptor(−) invasive ductal carcinoma, respectively. HETslide is computed for each individual replicate core of each patient and then computed for the entire patient (consolidating the cells from each core). HETROI is computed for each region of interest in the cores and the mean, minimum, and maximum HETROI scores are reported for each core and patient