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  Indian J Med Microbiol
 

Figure 5: Three-dimensional light-sheet microscopy image of a prostate biopsy measuring (2 cm in length by 1 mm in diameter). The biopsy specimen was chemically cleared with 2, 2' thiodiethanol to enable three-dimensional imaging, then stained with DRAQ5 (nuclear) and eosin (cytoplasmic) fluorescent dyes. A custom-built light-sheet microscope imaged the biopsy in three-dimensions. The total time for clarification, staining, and imaging was <20 min. The nuclear and cytoplasmic channels were false-colored and volume rendered using Imaris software

Figure 5: Three-dimensional light-sheet microscopy image of a prostate biopsy measuring (2 cm in length by 1 mm in diameter). The biopsy specimen was chemically cleared with 2, 2' thiodiethanol to enable three-dimensional imaging, then stained with DRAQ5 (nuclear) and eosin (cytoplasmic) fluorescent dyes. A custom-built light-sheet microscope imaged the biopsy in three-dimensions. The total time for clarification, staining, and imaging was <20 min. The nuclear and cytoplasmic channels were false-colored and volume rendered using Imaris software