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  Indian J Med Microbiol
 

Figure 3: Field of view of a slide stained with an immunofluorescence assay targeting CD8 (Cy5 - red), Ki67 (Cy3 - yellow), CD4 (FITC - green), CD3 (CFP - light blue), and nuclei (DAPI - dark blue). All fluorescence channels are combined in an RGB darkfield image. This type of images is still difficult to examine or annotate by pathologists

Figure 3: Field of view of a slide stained with an immunofluorescence assay targeting CD8 (Cy5 - red), Ki67 (Cy3 - yellow), CD4 (FITC - green), CD3 (CFP - light blue), and nuclei (DAPI - dark blue). All fluorescence channels are combined in an RGB darkfield image. This type of images is still difficult to examine or annotate by pathologists