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  Indian J Med Microbiol
 

Figure 4: Simplified process of obtaining virtual hematoxylin and eosin images from fluorescence images. We used 5 field of views from different slides to get the optimized transform parameters. We performed manual registration by selecting control points then fine-tuning by manual rotations and shifts to the single pixel accuracy

Figure 4: Simplified process of obtaining virtual hematoxylin and eosin images from fluorescence images. We used 5 field of views from different slides to get the optimized transform parameters. We performed manual registration by selecting control points then fine-tuning by manual rotations and shifts to the single pixel accuracy