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  Indian J Med Microbiol
 

Figure 1: Whole slide scans of lung cancer FFPE tissue section captured in 6 minutes. Top) Conventional narrowband scan acquired with bandpass filters optimal for Opal fluorophores. Bottom) Unmixed multispectral scan that removes crosstalk and autofluorescence. Arrows indicate autofluorescence contamination; asterisks indicate crosstalk from a spectrally adjacent band

Figure 1: Whole slide scans of lung cancer FFPE tissue section captured in 6 minutes. Top) Conventional narrowband scan acquired with bandpass filters optimal for Opal fluorophores. Bottom) Unmixed multispectral scan that removes crosstalk and autofluorescence. Arrows indicate autofluorescence contamination; asterisks indicate crosstalk from a spectrally adjacent band