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  Indian J Med Microbiol
 

Figure 1: Three phases of the analytic workflow for detecting, quantifying, and mapping proliferative cell nuclei (mitotic figure) in tissue whole-slide images. Initial phase involves creating grid tile regions of interest covering the entire tissue and extracting the necessary features to detect and segment phosphorylated histone H3-immunolabeled nuclei, a marker of proliferating cells in mitosis (M phase). In Phase II, the mitotic figure objects of interest are counted, and each grid tile tally is exported to a spreadsheet and rank ordered according to total counts. In the final conceptual Phase III, selected tiles (with greatest counts) are analyzed to obtain local features which can be mapped back to the whole slide image for display

Figure 1: Three phases of the analytic workflow for detecting, quantifying, and mapping proliferative cell nuclei (mitotic figure) in tissue whole-slide images. Initial phase involves creating grid tile regions of interest covering the entire tissue and extracting the necessary features to detect and segment phosphorylated histone H3-immunolabeled nuclei, a marker of proliferating cells in mitosis (M phase). In Phase II, the mitotic figure objects of interest are counted, and each grid tile tally is exported to a spreadsheet and rank ordered according to total counts. In the final conceptual Phase III, selected tiles (with greatest counts) are analyzed to obtain local features which can be mapped back to the whole slide image for display