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  Indian J Med Microbiol
 

Figure 6: Performance fit comparisons between repeated automated TMHS analyses and among a group of pathologists examining anti-phosphorylated histone H3-immunolabeled melanomas by conventional microscopy. (a) For TMHS, an original input image, with grid tile overlay, includes the computed TMHS h1 hotspot indicated by red box tile, which is illustrated in relation to the R1 hotspot selected by the pathologist (circle). (b) Identical image as in a, after 90° in-plane rotation, is processed in TMHS to demonstrate the computed h1 hotspot within the same tissue region as the original. The R1 hotspot, selected by the pathologist and shown as an image overlay, has the same tissue coordinates as in a. Bar = 1.514 mm. (c) Regression scatter plot of paired mitotic figure count values in the two image orientations among the 30 cases, from automated TMHS mapping tool (log10transformed). Blue-shaded area represents 95% confidence interval. R2 value and regression equation are indicated on the figure. Measurements from the two image orientations were highly significantly correlated (P < 0.0001). (d) Pair-wise, interobserver agreement of mitotic figure assessment by each pathologist using conventional microscopy on anti-phosphorylated histone H3-immunolabeled melanomas (n = 35). Each cell in the heat map represents the Spearman correlation coefficient (rho values, bar graph) between each pair of pathologists (A, B, F, J, K, and L), using the total mitotic figure count in ten high-power microscope fields of view

Figure 6: Performance fit comparisons between repeated automated TMHS analyses and among a group of pathologists examining anti-phosphorylated histone H3-immunolabeled melanomas by conventional microscopy. (a) For TMHS, an original input image, with grid tile overlay, includes the computed TMHS h1 hotspot indicated by red box tile, which is illustrated in relation to the R1 hotspot selected by the pathologist (circle). (b) Identical image as in a, after 90° in-plane rotation, is processed in TMHS to demonstrate the computed h1 hotspot within the same tissue region as the original. The R1 hotspot, selected by the pathologist and shown as an image overlay, has the same tissue coordinates as in a. Bar = 1.514 mm. (c) Regression scatter plot of paired mitotic figure count values in the two image orientations among the 30 cases, from automated TMHS mapping tool (log<sub>10</sub>transformed). Blue-shaded area represents 95% confidence interval. <i>R</i><sup>2</sup> value and regression equation are indicated on the figure. Measurements from the two image orientations were highly significantly correlated (<i>P</i> < 0.0001). (d) Pair-wise, interobserver agreement of mitotic figure assessment by each pathologist using conventional microscopy on anti-phosphorylated histone H3-immunolabeled melanomas (<i>n</i> = 35). Each cell in the heat map represents the Spearman correlation coefficient (rho values, bar graph) between each pair of pathologists (A, B, F, J, K, and L), using the total mitotic figure count in ten high-power microscope fields of view