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  Indian J Med Microbiol
 

Figure 3: Results of automated counts using different numbers of cells. In the example shown in (a), counting a field that included 1906 cells provided a Ki67 labeling index of 2.99%; in contrast, counting 1455 cells in the same area using a smaller region of interest resulted in a Ki67 labeling index of 3.09% for this tumor. Using the current WHO classification, the difference makes this either a G1 or a G2 tumor. In (b), counting 1421 cells provide a Ki67 of 19.49% and classification as a G2 tumor, whereas counting 992 cells results in a Ki67 of 21.27% and classification as a G3 tumor

Figure 3: Results of automated counts using different numbers of cells. In the example shown in (a), counting a field that included 1906 cells provided a Ki67 labeling index of 2.99%; in contrast, counting 1455 cells in the same area using a smaller region of interest resulted in a Ki67 labeling index of 3.09% for this tumor. Using the current WHO classification, the difference makes this either a G1 or a G2 tumor. In (b), counting 1421 cells provide a Ki67 of 19.49% and classification as a G2 tumor, whereas counting 992 cells results in a Ki67 of 21.27% and classification as a G3 tumor