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  Indian J Med Microbiol
 

Figure 1: Hematoxylin and eosin-stained image analyzed using the “cell detection” algorithm in QuPath (open-source tool). Nuclear segmentation, depicted as red outlines, was fragmented in the rightmost image by setting the noise reduction Gaussian filter σ = 1.0. Conversely, nuclear oversegmentation was achieved by setting σ = 2.5. Black arrows in the left image denote examples of single objects that consisted of multiple nuclei

Figure 1: Hematoxylin and eosin-stained image analyzed using the “cell detection” algorithm in QuPath (open-source tool). Nuclear segmentation, depicted as red outlines, was fragmented in the rightmost image by setting the noise reduction Gaussian filter σ = 1.0. Conversely, nuclear oversegmentation was achieved by setting σ = 2.5. Black arrows in the left image denote examples of single objects that consisted of multiple nuclei