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  Indian J Med Microbiol
 

Figure 4: Digital pathology image analysis in spatial context reveal biomarker and cell heterogeneity. (a) The inset digital slide with DAB-stained biomarker (brown) was analyzed. Cells identified in the analysis were plotted spatially as a dot plot and each cell “dot” color coded according to the optical density of DAB stain in that cell. Cells in “cooler” colors (blues and greens) have lower stain optical density compared to cells in “warmer” colors (yellow, orange, and red). (b) Tumor cells and immune cells (DAB-positive) identified by image analysis were plotted spatially and analyzed to quantify immune cells within 30 μm of tumor cells (proximal immune cells). Tumor cells are colored blue, proximal immune cells are colored red, and nonproximal immune cells are green. The distance between tumor cells and proximal immune cells are recorded to create a histogram (inset, bottom right) and are connected by nearest neighbor lines in the dot plot

Figure 4: Digital pathology image analysis in spatial context reveal biomarker and cell heterogeneity. (a) The inset digital slide with DAB-stained biomarker (brown) was analyzed. Cells identified in the analysis were plotted spatially as a dot plot and each cell “dot” color coded according to the optical density of DAB stain in that cell. Cells in “cooler” colors (blues and greens) have lower stain optical density compared to cells in “warmer” colors (yellow, orange, and red). (b) Tumor cells and immune cells (DAB-positive) identified by image analysis were plotted spatially and analyzed to quantify immune cells within 30 μm of tumor cells (proximal immune cells). Tumor cells are colored blue, proximal immune cells are colored red, and nonproximal immune cells are green. The distance between tumor cells and proximal immune cells are recorded to create a histogram (inset, bottom right) and are connected by nearest neighbor lines in the dot plot