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  Indian J Med Microbiol
 

Figure 5: Digital pathology image analysis in the pancreas and brain. (a) Islets stained with antibodies against insulin (red stain) and glucagon (brown stain) in digital slides. Analysis shown bottom left quantifies number of islets that are of islet (orange area in markup) and number of cells that are positive for insulin (red cell markup), glucagon (green cell markup), both (yellow cell markup), or neither marker (white cell markup). (b) Identification of beta-amyloid plaques in brain sections. Slides are probed with antibodies against beta-amyloid (purple) and vessel endothelial marker (brown). Digital image analysis shown bottom right quantifies density, diameter, and area of vessels (red markup) and plaques (green markup), and colocalized area (yellow markup)

Figure 5: Digital pathology image analysis in the pancreas and brain. (a) Islets stained with antibodies against insulin (red stain) and glucagon (brown stain) in digital slides. Analysis shown bottom left quantifies number of islets that are of islet (orange area in markup) and number of cells that are positive for insulin (red cell markup), glucagon (green cell markup), both (yellow cell markup), or neither marker (white cell markup). (b) Identification of beta-amyloid plaques in brain sections. Slides are probed with antibodies against beta-amyloid (purple) and vessel endothelial marker (brown). Digital image analysis shown bottom right quantifies density, diameter, and area of vessels (red markup) and plaques (green markup), and colocalized area (yellow markup)