Journal of Pathology Informatics Journal of Pathology Informatics
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Year : 2011  |  Volume : 2  |  Issue : 1  |  Page : 48

Standardization of whole slide image morphologic assessment with definition of a new application: Digital slide dynamic morphometry

1 Division of Pathology, G. Fracastoro, City Hospital, Verona; PhD Programme in 2 Experimental Medicine and Oncology, University of Insubria, Varese, Italy
2 Unit of Pathology, Institute for Cancer Research and Treatment-IRCC, Candiolo, Torino, Italy
3 Centre for Colorectal Disease, St. Vincent's University Hospital School of Medicine and Medical Science, University College Dublin, Dublin, Ireland
4 Institute of Pathology, Klinikum Bayreuth, Bayreuth, Germany
5 Institute for Pathology, University Hospital Basel, Basel, Switzerland
6 Department of Pathology, Section of Anatomical Pathology, Policlinico G. B. Rossi, University of Verona, Italy
7 Department of Cellular Pathology, John Radcliffe Hospital, Headington, Oxford, United Kingdom
8 Institute of Pathology, Medical University of Graz, Graz, Austria
9 Department of Human Pathology, Juntendo University School of Medicine, Tokyo, Japan
10 Department of Surgery, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan
11 Department of Surgery, National Defense Medical College, Namiki, Tokorozawa, Saitama, Japan
12 Department of Pathology, François Baclesse Comprehensive Cancer Center, Caen, France
13 AOUS Giovanni Battista - CPO Piemonte, SCDO Epidemiologia dei Tumori, Torino, Italy
14 Pathology Media Lab, Pathology Service, Massachusetts General Hospital, Boston, MA, USA
15 Groupe Régional d'Etudes sur le Cancer, University of Caen, François Baclesse Comprehensive Cancer Center, Caen, France
16 Gastrointestinal Pathology Service and Division of Surgical Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA

Correspondence Address:
Giacomo Puppa
Division of Pathology, G. Fracastoro, City Hospital, Verona; PhD Programme in 2 Experimental Medicine and Oncology, University of Insubria, Varese
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2153-3539.86830

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Background: In histopathology, the quantitative assessment of various morphologic features is based on methods originally conceived on specific areas observed through the microscope used. Failure to reproduce the same reference field of view using a different microscope will change the score assessed. Visualization of a digital slide on a screen through a dedicated viewer allows selection of the magnification. However, the field of view is rectangular, unlike the circular field of optical microscopy. In addition, the size of the selected area is not evident, and must be calculated. Materials and Methods: A digital slide morphometric system was conceived to reproduce the various methods published for assessing tumor budding in colorectal cancer. Eighteen international experts in colorectal cancer were invited to participate in a web-based study by assessing tumor budding with five different methods in 100 digital slides. Results: The specific areas to be tested by each method were marked by colored circles. The areas were grouped in a target-like pattern and then saved as an .xml file. When a digital slide was opened, the .xml file was imported in order to perform the measurements. Since the morphometric tool is composed of layers that can be freely moved on top of the digital slide, the technique was named digital slide dynamic morphometry. Twelve investigators completed the task, the majority of them performing the multiple evaluations of each of the cases in less than 12 minutes. Conclusions: Digital slide dynamic morphometry has various potential applications and might be a useful tool for the assessment of histologic parameters originally conceived for optical microscopy that need to be quantified.

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