|SYMPOSIUM - ORIGINAL RESEARCH
|J Pathol Inform 2011,
Atlas-guided correction of brain histology distortion
Xi Qiu1, Lin Shi2, Tony Pridmore3, Alain Pitiot3, Defeng Wang2
1 Rotman Research Institute, University of Toronto, Canada; Department of Imaging and Interventional Radiology, Chinese University of Hong Kong, China; and School of Computer Science, University of Nottingham, UK
2 Department of Imaging and Interventional Radiology, Chinese University of Hong Kong, China
3 School of Computer Science, University of Nottingham, UK
|Date of Submission||15-Jun-2011|
|Date of Acceptance||23-Sep-2011|
|Date of Web Publication||19-Jan-2012|
Rotman Research Institute, University of Toronto, Canada; Department of Imaging and Interventional Radiology, Chinese University of Hong Kong, China; and School of Computer Science, University of Nottingham, UK
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Histological tissue preparation stages (e.g., cutting, sectioning, etc.) often introduce tissue distortions that prevent a smooth 3D reconstruction from being built. In this paper, we propose a method to correct histology distortions by running a piecewise registration scheme. It takes the information of several consecutive slices in a neighborhood into account. In order to achieve an accurate anatomic presentation, we run the method iteratively with the assistance from a pre-segmented brain atlas. The registration parameters are optimized to accommodate different brain sub-regions, e.g., cerebellum, hippocampus, etc. The results are evaluated by both visual and quantitative approaches. The proposed method has been proved to be robust enough for reconstructing an accurate and smooth mouse brain volume.
Keywords: Piecewise image registration, Histology volume reconstruction, Histology distortion correction, Atlas-guided correction
|How to cite this article:|
Qiu X, Shi L, Pridmore T, Pitiot A, Wang D. Atlas-guided correction of brain histology distortion. J Pathol Inform 2011;2, Suppl S1:7
| Introduction|| |
Building and studying 3D representations of anatomical organs, such as the brain, plays an important role in modern biology and medical science. While 3D imaging methods such as MRI and CT provide accurate 3D structural information, 2D imaging methods such as histology and optical microscopy typically generate images with much higher resolution and better specific contrast. When studying the mouse brain, it is ideal to combine the advantages of both 3D and 2D imaging technologies. The classical approach is to reconstruct a 3D mouse brain volume from a series of histology slice images that provide more tissue details than MR images. ,, However, histology acquisition generally induces a lot of artifacts (holes, folding, tearing, sketching, etc.). Detecting and correcting the artifacts becomes a central issue when reconstructing a 3D volume from a series of histology slices. Indeed, they can make the distorted regions significantly different from the corresponding regions in adjacent slices. In a typical pairwise registration approach, artifacts lead to registration errors that tend to propagate to the adjacent slices through the slice-by-slice registration so that it prevents a smooth 3D brain volume from being reconstructed. Therefore, postacquisition distortion corrections are necessary. , As discussed, a conventional slice-by-slice 2D registration is not sufficient to correct the histology distortions.  In this case, 3D registration techniques can be employed to overcome the problem. ,,,
Since studies in region of interest of mouse brain have been very popular in the past several decades, many 3D Atlas More Detailses are made available to support the research. ,,, Mostly, they were acquired by one of the 3D imaging techniques (e.g., MRI, CT), and then manually segmented by biologists. The resolution of those atlases varies a lot due to the different acquisition means and settings. The details of segmentations also differ a lot based on the degree of how small the functional regions in brain that the researchers required. Because the segmentation of the regions of interest in the atlas is reasonably closed to the ground truth, ,, it can be used as very supportive information to improve the quality and the accuracy of histological reconstruction.
A 3D volume reference acquired from the same brain of histology slices before sectioning would be perfect to correct the anatomically wrong information. However, such reference is not always available due to lack of equipment, limit of project, etc. While mouse brain anatomic atlas has been developed maturely, atlas from the same strain may not be the same individual as the histology sections, but still reasonably similar, hence can be used as a 3D volume reference. Moreover, atlases are well labeled with clear delineations of anatomical components that can lead us to correct the anatomical structures and shapes more locally and more purposefully.
Researchers have proposed approaches to detect distorted slices by evaluating the quality of image registration between slices.  Other methods , are based on the idea of eliminating the distorted slices rather than correcting them where possible. Quite often, most of serial histology slices have different types of distortions in different regions. If they are all removed, there may be not sufficient information left to reconstruct a 3D volume. A framework  was proposed to detect the distorted slices and predict the possible distorted structures while it may not be robust enough for a large amount of distortions. Therefore, we developed a method that takes more local information into account in a piecewise fashion and borrows information from a 3D atlas to improve the accuracy and robustness of distortion correction.
| Methodology|| |
Piecewise correction of histology distortion
In this method, we register histology slice S i 's neighbors located up to m slices away to itself using block-matching registration technique,  provided the slice thickness is small and even. Then, the local corresponding displacements (r: region of interest; j = i+n; n = 1, 2, ...,m) across those m slices are put together for comparison as shown in [Figure 1]. If the structure is not distorted and follows a smooth transition across several consecutive sections, the corresponding displacement vectors should have similar directional trends and similar lengths. However, if there is a structural distortion in at least one slice, the displacement vector of the distorted region in that slice will differ in direction and/or length from its counterpart vectors. Consequently, our target is to find those significantly different vectors so that we can have the knowledge of in which slice and where in that slice there may be a distortion, and also how the distortion may look like.
|Figure 1: Displacements of the corresponding regions before and after weighted averaging|
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To make a fair comparison of the vectors, we employ a Gaussian filter to weight the distance n between the floating images and the reference image. The vector of interest receives the heaviest weight and the displacements of the corresponding regions in the neighboring slices receive smaller weights as their distance n to slice S i increases, i.e.
where σ is standard deviation of Gaussian distribution.
In addition to the Gaussian filter, averaged similarity measure CC (correlation coefficient) of the ROI has also been taken into account to weight the displacements. The regions that are more similar to the ROI receive higher weights, and vice versa:
where m is the number of the slices in this iteration.
Therefore, by combining the two terms of weights, we then derive a new set of displacements for the ROI r as shown in [Figure 1]:
Outlier detection using Z-score testing
Our outlier detection is based on standardized Z-score testing, i.e., Therefore,
where is the transformation in region r at slice i; and are the mean and the standard deviation of the displacements in region r at slice i to j, respectively.
In order to achieve good accuracy, we set the confidence level to 95%, and consequently, the Z = 1.64.
After the Z-score test, we identify the transformation that significantly differs from the others. Based on our theory, the ROI in that slice is very likely to be distorted. We should run our piecewise correction on that region.
Registration of outlier to nonoutlier regions
For every outlier region r outlier found in the last step, we apply the block-matching algorithm again to warp r outlier to its neighboring slices. For example, if S i+2 in [Figure 1] is found to have outlier region, the estimated displacement for the outlier region is
Here, we assume droutlier is invertible, i.e. droutlier = (d-1routlier )-1
Therefore, by applying the inverted displacement to the outlier region, we make the outlier regions more similar to its nonoutlier neighbors, e.g., in [Figure 2]. We then implement this algorithm to every outlier region found within the slice neighborhood to correct histology distortions.
|Figure 2: The distortion (dot-line square) in Si+2 is corrected by applying the inverted displacement of (5)|
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Our algorithm also runs iteratively to cover all the histology slices. In each iteration, we treat m consecutive slices S i ~S i+m-1 as a registration neighborhood and choose the first slice of those m ones as the reference image. In the next iteration, neighborhood window shifts by one slice, i.e., S i+1 ~S i+m . By this way, every slice is treated as the reference of a group of consecutive slices to avoid reference bias.
Displacement regularization and filtering
Inevitably, a fair amount of outlier displacements might be generated by registration error. They may not match the anatomical features of interest adequately. An adaptive displacement regularization approach  estimates a rigid transformation within a predefined circular neighborhood cut to fit the local geometry around the region of interest and for each surrounding region that a displacement has been computed. Inhomogeneous displacements caused by registration error are regularized by this rigid transformation.
To make the whole displacement field even smoother, a mean filter is also applied in a circular neighborhood around every region of interest. The filter averages displacements in both x and y directions:
where j is the regions within the circular neighborhood around region of interest.
Finally, we warp every slice using the regularized and filtered displacement fields. All the corrected slices are then registered pair by pair to form a volume.
Atlas-guided anatomic structure recovery
A mouse brain has been reconstructed in the previous section. Nevertheless, the fact of lack of support and proof from a 3D ground truth still prevents the reconstructed volume from becoming a solid foundation for future studies. Therefore, a 3D reference is still necessary here for correcting the shape of reconstruction and, much expectantly, improving local smoothness as well. Consequently, we introduce a structural-improving technique of reconstructed volume guided by a presegmented 3D atlas of mouse brain. The already reconstructed volume is fused with the atlas. In each brain subregion, we further refine the parameters of our piecewise distortion correction method to achieve accurate brain structure and better volume smoothness.
- Register the 3D brain atlas of the same mouse strain to our reconstruction by the 3D fusion technique. 
- Warp the atlas labels to the space of our reconstruction by the transformation of 1.
- Label the brain regions in the reconstruction according to the warped atlas segmentation.
- Refine the parameters for distortion correction to adapt the different brain regions.
- Reconstruct a new volume using the refined parameters.
The method is operated in a coarse-to-fine fashion. The above steps are looped until a stopping criterion has been met. In the first iteration, a set of initial parameters is assigned for step 3. Those parameters are recommended as a prior knowledge by our neurologists based on the atlas. Thereafter, all the following iterations halve the parameters from the last iteration. We implement the method in this way to ensure the parameters can be optimized rapidly. The stopping criterion is defined as an evaluation of mean smoothness  of the reconstructed volume, i.e., when mean smoothness measure S k≤1 , we stop the loop and output the reconstruction as the final result.
| Experimental Results|| |
Experiment setup and parameters
In our experiment, a set of 350 Nissl-stained coronal images acquired by cyro-sectioning a single frozen C57BL/6J adult mouse brain from LONI Research Lab at the UCLA was used. Each image was sized to 900 × 900 pixels in a resolution of 10 μm per pixel during acquisition. The distance between the consecutive sections is 25 μm. In order to reduce the registration errors, we ran a prior step by applying a Gaussian filter (σ = 3) on the images to downsize them and reduce noise.
The computation of 87 iterations consumed a total ~40 hours, averaging ~15 minutes for the local correction of each slice. The block size of 1111, lattice site of every 10 pixels, and exploration neighborhood size of 71×71 were chosen, which was sufficient to cover the misaligning range for different slices in the dataset. Geometrical rigid regularization was applied on a circular range with a radius of 50 pixels and followed by an averaging filter with a radius of 20 pixels to optimize the raw displacement fields derived by the block-matching registration.
Based on the synthesized consideration of all the atlas' features,  the MR C57BL/6J Mouse Brain Atlas built by Brookhaven National Laboratory (BNL) was chosen as the 3D reference in our study.
2D correction results
The tuning regularization radius R (0~∞) will change the displacement field significantly. When R = 0, the geometrical regularization takes no effect on the displacement field, which equals a fully flexible transformation; whereas when R = ∞), a global rigid transformation will be yielded. As the R value becomes larger, the displacements become lighter and more evened out. Based on the resolution of our dataset and the minimum size of the topological texture we wanted to recover, we chose R=50 pixels as the radius of the regularization circular neighborhood.
After the displacement field had been calculated for outlier correction, we applied a serious of postprocessing steps to the displacement field in order. Parameters were regularization radius=50, averaging filter radius=20, and bilinear interpolation. Clearly, the corrected consecutive slices were locally aligned much better in details as highlighted in [Figure 3]. The better alignment would increase the reconstruction quality significantly.
|Figure 3: Distortion correction results in 2D: Two consecutive slices (no. 138 in red and no. 139 in green; perfect alignments: yellow) superimposed: (a) before and (b) after the piecewise distortion correction|
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3D reconstruction results
The chosen BNL atlas volume was registered to our reconstruction result from the method described in the Piecewise Correction of Histology Distortion section. The derived transformation was then applied to warp the atlas labels to the reconstruction. As [Figure 4] shows, the main anatomical brain regions have been labelled in our reconstructed volume.
|Figure 4: Warped atlas labels superimposed on top of the reconstructed volume|
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In our experiment, parameters were halved every iteration to quickly regress to the optimized combination. [Table 1] shows the final set of the optimized parameters.
In [Figure 5], the hippocampus reconstructed by the optimized parameters in [Table 1] shows the accurate anatomic structure and the smoothest texture among the four.
|Figure 5: Hippocampus reconstructions in Sagittal view: (a) rigid-body alignment; (b) piecewise distortion correction only; (c) atlas-guided correction based on the initial parameters; (d) atlas-guided correction based on the optimized parameters|
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A further noise removing tool - majority filter  - can be employed to ensure a smoother appearance of the reconstructed volume in the applications where intensity inhomogeneity is zero-tolerant, e.g., high-resolution visualization and atlas building.
To clearly show the advantages and the possible drawbacks of our atlas-guided scheme, we compared our results in [Figure 6] (c) with Guest's (a), Ju's (b) corresponding views of results and also Paxino's histology atlas  as a reference.
|Figure 6: Result comparisons in axial and sagittal views: (a, b) reconstructed volume[3,18]; (c) our result; (d) no. 157 axial and no. 110 sagittal section of Paxino's atlas|
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Among the three sets of results, our reconstructed volume was noted that the inside anatomical features became a lot more coherent. Moreover, the result showed that the shapes of many key structures had been recovered thanks to the scheme of labeling out the brain regions and dealing with them in a respective manner. Our result was found to have more matches of anatomical regions with Paxino's atlas compared to either Guest's or Ju's results, in particular, hippocampus, corpus callosum, and cerebellum.
To evaluate the quality of smoothness achieved in our reconstructed volume, we employed a smoothness measure  Sk for every slice k as the following:
where A (i,j) denotes the location of (i,j) in the warped image under evaluation; B(i,j) and C(i,j) denote the locations of the corresponding pixels of (i,j) on the two neighboring slices, respectively. Sk acts similar to the CAM measure. 
By the definition, Sk reflects the average distance from each pixel A0 (i,j) to the mid-point of its two corresponding pixels in the consecutive slices, i.e.,
In other words, in the reconstructed volume, each pixel deviates averagely from the middle of its two corresponding pixels on the neighboring slices by (B(i,j) + C(i,j)-2A(i,j)) pixels.
To ensure that our reconstructed volume has achieved the satisfaction quantitatively, we computed a smoothness evaluation Sk which was also used as the stopping criterion in our iterative program. With the optimized parameters and majority filtering of three sections on each side, we achieved a reconstruction with a mean smoothness evaluation Sk = 0.92, i.e., in the reconstructed volume, on average, each point deviates from the middle of its two corresponding pixels on the neighboring sections by only about 0.46 pixel (9.8% closer than Ju's result: 0.51 pixel  ).
| Discussion and Conclusion|| |
In this paper, we proposed a piecewise registration scheme and an atlas-guided anatomy recovery for mouse brain reconstruction. 2D distortions in histology slice were corrected in some degrees with the help from the undistorted information from the slice's neighbors. Presegmented atlas labels were firstly warped to the space of our reconstructed volume after the piecewise registration. Labeled anatomical regions in our reconstruction were then assigned an initial set of parameters of the piecewise registration and the optimization steps by neurologists based on the anatomical feature of the individual brain regions. Our automated framework was then operated to optimize the parameters iteratively by correcting local distortion piecewisely and rewarping the corrected sections pairwisely.
For result inspection, both visual and quantitative evaluations had been performed in comparison with other competitive approaches. Despite lacking global alignment in some regions compared to other methods due to the local nature of the atlas labels we referenced to, our results still showed clear advantages of local smoothness and better matching with the real histology sections over other methods in the comparison. Moreover, our method requires the least manual manipulation in the comparison.
In summary, our method has been proved as a reliable and robust way to correct distortions of local regions across consecutive histology sections and to subsequently reconstruct a smooth volume of mouse brain.
| Acknowledgment|| |
The work described in this paper was partially supported by a direct grant from CUHK Research Committee Funding, the Hong Kong Special Administrative Region, China (Project No.: MD10581).
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[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6]