Journal of Pathology Informatics Journal of Pathology Informatics
Contact us | Home | Login   |  Users Online: 85  Print this pageEmail this pageSmall font sizeDefault font sizeIncrease font size 


RESEARCH ARTICLE
Year : 2020  |  Volume : 11  |  Issue : 1  |  Page : 35

Computerized image analysis of tumor cell nuclear morphology can improve patient selection for clinical trials in localized clear cell renal cell carcinoma


1 School of Medicine, University of St Andrews, St Andrews, Scotland
2 School of Mathematics and Statistics, University of St Andrews, St Andrews, Scotland
3 Department of Pathology, Singapore General Hospital, Singapore
4 Division of Medical Oncology, National Cancer Centre, Singapore
5 Institute of Bioengineering and Nanotechnology, Singapore
6 Lucence Diagnostics Pte Ltd, Singapore
7 Department of Pathology, Western General Hospital, Edinburgh, Scotland
8 Department of Urology, Western General Hospital, Edinburgh, Scotland
9 Department of Surgery, University of Cambridge, Cambridge, England
10 School of Medicine, University of St Andrews and Lothian NHS University Hospitals, St Andrews, Scotland

Correspondence Address:
Dr. In Hwa Um
School of Medicine, University of St Andrews, St Andrews
Scotland
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jpi.jpi_13_20

Rights and Permissions

Background: Clinicopathological scores are used to predict the likelihood of recurrence-free survival for patients with clear cell renal cell carcinoma (ccRCC) after surgery. These are fallible, particularly in the middle range. This inevitably means that a significant proportion of ccRCC patients who will not develop recurrent disease enroll into clinical trials. As an exemplar of using digital pathology, we sought to improve the predictive power of “recurrence free” designation in localized ccRCC patients, by precise measurement of ccRCC nuclear morphological features using computational image analysis, thereby replacing manual nuclear grade assessment. Materials and Methods: TNM 8 UICC pathological stage pT1-pT3 ccRCC cases were recruited in Scotland and in Singapore. A Leibovich score (LS) was calculated. Definiens Tissue studio® (Definiens GmbH, Munich) image analysis platform was used to measure tumor nuclear morphological features in digitized hematoxylin and eosin (H&E) images. Results: Replacing human-defined nuclear grade with computer-defined mean perimeter generated a modified Leibovich algorithm, improved overall specificity 0.86 from 0.76 in the training cohort. The greatest increase in specificity was seen in LS 5 and 6, which went from 0 to 0.57 and 0.40, respectively. The modified Leibovich algorithm increased the specificity from 0.84 to 0.94 in the validation cohort. Conclusions: CcRCC nuclear mean perimeter, measured by computational image analysis, together with tumor stage and size, node status and necrosis improved the accuracy of predicting recurrence-free in the localized ccRCC patients. This finding was validated in an ethnically different Singaporean cohort, despite the different H and E staining protocol and scanner used. This may be a useful patient selection tool for recruitment to multicenter studies, preventing some patients from receiving unnecessary additional treatment while reducing the number of patients required to achieve adequate power within neoadjuvant and adjuvant clinical studies.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed280    
    Printed4    
    Emailed0    
    PDF Downloaded33    
    Comments [Add]    

Recommend this journal