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  Indian J Med Microbiol
 

Figure 1: How whole-slide imaging changes the workflow in pathology. Conventionally, a glass slide is viewed by the human reader through a light microscope, which processes the image in the optical domain (cyan shaded) entirely and does not impose color performance issues. In digital pathology, a whole-slide imaging system uses a scanner to convert the optical image into the digital domain (yellow shaded) with computer hardware and software components. The image data are stored in a specific file format and transmitted to the review workstation. The review workstation decodes the digital data and reproduces the image in the optical domain with a display. The color spaces used by the scanner, file format, and display determine whether the histological image can be faithfully reproduced

Figure 1: How whole-slide imaging changes the workflow in pathology. Conventionally, a glass slide is viewed by the human reader through a light microscope, which processes the image in the optical domain (cyan shaded) entirely and does not impose color performance issues. In digital pathology, a whole-slide imaging system uses a scanner to convert the optical image into the digital domain (yellow shaded) with computer hardware and software components. The image data are stored in a specific file format and transmitted to the review workstation. The review workstation decodes the digital data and reproduces the image in the optical domain with a display. The color spaces used by the scanner, file format, and display determine whether the histological image can be faithfully reproduced